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TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. In molecular biology it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA.〔Ogden, R.C., and Adams, D.A., (1987) Electrophoresis in agarose and acrylamide gels. ''Methods Enzymol''., 152:, 61-87.〕 It is made up of Tris-acetate buffer, usually at pH 8.0, and EDTA, which sequesters divalent cations. TAE has a lower buffer capacity than TBE and can easily become exhausted, but linear, double stranded DNA runs faster in TAE. Recently, Brody & Kern simplified electrophoretic buffers by substituting TBE and TAE buffers for a more efficient and inexpensive conductive media in gel systems.〔Brody, J.R., Kern, S.E. (2004) History and principles of conductive media for standard DNA electrophoresis. ''Anal Biochem.'' 333(1):1-13. PMID 15351274 (PDF )〕 ==Uses== TAE (Tris-acetate-EDTA) buffer is used as both a running buffer and in agarose gel.〔Sambrook, Fritsch, and Maniatis (1989) ''Molecular Cloning: A Laboratory Manual'', 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, volume 3, apendices B.11 and B.23 ISBN 0-87969-309-6〕 Its use in denaturing gradient gel electrophoresis methods for broad-range mutation analysis has also been described.〔Hayes, V.M. et al., (1999) Improvements in gel composition and electrophoretic conditions for broad-range mutation analysis by denaturing gradient gel electrophoresis. ''Nucleic Acids Res''., 27(20): e29. PMID 10497279〕 TAE has been used at various concentrations to study the mobility of DNA in solution with and without sodium chloride.〔Stellwagen, E., and Stellwagen, N.C. (2002) The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl. ''Electrophoresis'', 23(12): 1935-1941. PMID 12116139〕 However, high concentrations of sodium chloride (and many other salts) in a DNA sample retard its mobility. This may lead to incorrect interpretations of the resulting DNA banding pattern. Compared with TBE buffer, TAE buffer offers advantages in subsequent enzymatic applications for the DNA sample. For example, if a DNA sample is going to be used in a cloning experiment, the step that follows its running on an agarose gel is to ligate (covalently link) to a cloning vector (most likely a plasmid). A DNA sample from a TAE gel is suitable for this purpose, while DNA from a TBE gel is not, because borate in the TBE buffer is a strong inhibitor for many enzymes . 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「TAE buffer」の詳細全文を読む スポンサード リンク
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